Ontology highlight
ABSTRACT:
SUBMITTER: Walter J
PROVIDER: S-EPMC91821 | biostudies-literature | 2000 Jan
REPOSITORIES: biostudies-literature
Walter J J Tannock G W GW Tilsala-Timisjarvi A A Rodtong S S Loach D M DM Munro K K Alatossava T T
Applied and environmental microbiology 20000101 1
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR primers that targeted 16S-23S rRNA intergenic spacer region or 16S rRNA gene sequences. The identifica ...[more]