Ontology highlight
ABSTRACT:
SUBMITTER: Lai JP
PROVIDER: S-EPMC262429 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
Lai Jian-Ping JP Yang Ji-Hong JH Douglas Steven D SD Wang Xu X Riedel Eric E Ho Wen-Zhe WZ
Clinical and diagnostic laboratory immunology 20031101 6
CCR5, a beta-chemokine receptor, plays an important role in human immunodeficiency virus (HIV) infection of human immune cells, as it is a primary coreceptor for HIV entry into macrophages. We have applied a newly developed real-time reverse transcriptase PCR (RT-PCR) assay for the quantification of CCR5 mRNA in human blood immune cells. The CCR5 real-time RT-PCR assay has a sensitivity of 100 mRNA copies, with a dynamic range of detection between 10(2) and 10(6) copies of the CCR5 mRNA transcri ...[more]