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Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.

ABSTRACT: The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with comparable sensitivities. Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities.

SUBMITTER: Maertzdorf J

PROVIDER: S-EPMC356857 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

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Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.

Maertzdorf Jeroen J Wang Chiaoyin K CK Brown Jennifer B JB Quinto Joseph D JD Chu Marla M de Graaf Miranda M van den Hoogen Bernadette G BG Spaete Richard R Osterhaus Albert D M E AD Fouchier Ron A M RA

Journal of clinical microbiology 20040301 3

The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with compa ...[more]

PMID: 15004041

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