Ontology highlight
ABSTRACT:
SUBMITTER: Liu OW
PROVIDER: S-EPMC2628477 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Liu Oliver W OW Chun Cheryl D CD Chow Eric D ED Chen Changbin C Madhani Hiten D HD Noble Suzanne M SM
Cell 20081001 1
The fungus Cryptococcus neoformans is a leading cause of mortality and morbidity among HIV-infected individuals. We utilized the completed genome sequence and optimized methods for homologous DNA replacement using high-velocity particle bombardment to engineer 1201 gene knockout mutants. We screened this resource in vivo for proliferation in murine lung tissue and in vitro for three well-recognized virulence attributes-polysaccharide capsule formation, melanization, and growth at body temperatur ...[more]