Ontology highlight
ABSTRACT:
SUBMITTER: Hirrlinger J
PROVIDER: S-EPMC2628726 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Hirrlinger Johannes J Scheller Anja A Hirrlinger Petra G PG Kellert Beate B Tang Wannan W Wehr Michael C MC Goebbels Sandra S Reichenbach Andreas A Sprengel Rolf R Rossner Moritz J MJ Kirchhoff Frank F
PloS one 20090127 1
Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split-Cre ...[more]