Reductive dioxygen scavenging by flavo-diiron proteins of Clostridium acetobutylicum.
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ABSTRACT:
SUBMITTER: Hillmann F
PROVIDER: S-EPMC2629652 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
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Reductive dioxygen scavenging by flavo-diiron proteins of Clostridium acetobutylicum.
Hillmann Falk F Riebe Oliver O Fischer Ralf-Jörg RJ Mot Augustin A Caranto Jonathan D JD Kurtz Donald M DM Bahl Hubert H
FEBS letters 20081211 1
Two flavo-diiron proteins (FDPs), FprA1 and FprA2, are up-regulated when the strictly anaerobic solvent producer, Clostridium acetobutylicum, is exposed to dioxygen. These two FDPs were purified following heterologous overexpression in Escherichia coli as N-terminal Strep-tag fusion proteins. The recombinant FprA1 and FprA2 were found to be homodimeric and homotetrameric, respectively, and both FDPs functioned as terminal components of NADH oxidases (NADH:O(2) oxidoreductases) when using C. acet ...[more]
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