Ontology highlight
ABSTRACT:
SUBMITTER: Anbanandam A
PROVIDER: S-EPMC2632303 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Anbanandam Asokan A Albarado Diana C DC Tirziu Daniela C DC Simons Michael M Veeraraghavan Sudha S
Journal of molecular biology 20080916 1
PR39, a naturally occurring and cell-permeable proline- and arginine-rich peptide, blocks the degradation of inhibitor of nuclear factor kappaB (IkappaBalpha), thereby attenuating inflammation. It is a noncompetitive and reversible inhibitor of 20S proteasome. To identify its basis of action, we used solution NMR spectroscopy and mutational analyses of the active fragment, PR11, which identified amino acids required for human 20S proteasome inhibiting activity. We then examined PR11-mediated cha ...[more]