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A rainbow of fluoromodules: a promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes.

ABSTRACT: Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar Kd values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering most of the visible and near-IR regions of the spectrum. This significantly expands the number and wavelength range of scFv-based fluoromodules.

SUBMITTER: Ozhalici-Unal H

PROVIDER: S-EPMC2633110 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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A rainbow of fluoromodules: a promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes.

Ozhalici-Unal Hayriye H Pow Crystal Lee CL Marks Sarah A SA Jesper Lawrence D LD Silva Gloria L GL Shank Nathaniel I NI Jones Elizabeth W EW Burnette James M JM Berget Peter B PB Armitage Bruce A BA

Journal of the American Chemical Society 20080830 38

Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar Kd values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering mo ...[more]

PMID: 18761447

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