Ontology highlight
ABSTRACT:
SUBMITTER: Yu TY
PROVIDER: S-EPMC2633174 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Yu Tsyr-Yan TY Schaefer Jacob J
Journal of molecular biology 20080805 4
Bacteriophage T4 is a large-tailed Escherichia coli virus whose capsid is 120x86 nm. ATP-driven DNA packaging of the T4 capsid results in the loading of a 171-kb genome in less than 5 min during viral infection. We have isolated 50-mg quantities of uniform (15)N- and [epsilon-(15)N]lysine-labeled bacteriophage T4. We have also introduced (15)NH(4)(+) into filled, unlabeled capsids from synthetic medium by exchange. We have examined lyo- and cryoprotected lyophilized T4 using (15)N{(31)P} and (31 ...[more]