Ontology highlight
ABSTRACT:
SUBMITTER: Vafabakhsh R
PROVIDER: S-EPMC4210281 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Vafabakhsh Reza R Kondabagil Kiran K Earnest Tyler T Lee Kyung Suk KS Zhang Zhihong Z Dai Li L Dahmen Karin A KA Rao Venigalla B VB Ha Taekjip T
Proceedings of the National Academy of Sciences of the United States of America 20141006 42
Viral DNA packaging motors are among the most powerful molecular motors known. A variety of structural, biochemical, and single-molecule biophysical approaches have been used to understand their mechanochemistry. However, packaging initiation has been difficult to analyze because of its transient and highly dynamic nature. Here, we developed a single-molecule fluorescence assay that allowed visualization of packaging initiation and reinitiation in real time and quantification of motor assembly a ...[more]