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Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools.


ABSTRACT: Clathrin is a coat protein involved in vesicle budding from several membrane-bound compartments within the cell. Here we present an analysis of a temperature-sensitive (ts) mutant of clathrin heavy chain (CHC) in a multicellular animal. As expected Caenorhabditis elegans chc-1(b1025ts) mutant animals are defective in receptor-mediated endocytosis and arrest development soon after being shifted to the restrictive temperature. Steady-state clathrin levels in these mutants are reduced by more than 95% at all temperatures. Hub interactions and membrane associations are lost at the restrictive temperature. chc-1(b1025ts) animals become paralyzed within minutes of exposure to the restrictive temperature because of a defect in the nervous system. Surprisingly synaptic vesicle number is not reduced in chc-1(b1025ts) animals. Consistent with the normal number of vesicles, postsynaptic miniature currents occur at normal frequencies. Taken together, these results indicate that a high level of CHC activity is required for receptor-mediated endocytosis in nonneuronal cells but is largely dispensable for maintenance of synaptic vesicle pools.

SUBMITTER: Sato K 

PROVIDER: S-EPMC2633560 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools.

Sato Ken K   Ernstrom Glen G GG   Watanabe Shigeki S   Weimer Robby M RM   Chen Chih-Hsiung CH   Sato Miyuki M   Siddiqui Ayesha A   Jorgensen Erik M EM   Grant Barth D BD  

Proceedings of the National Academy of Sciences of the United States of America 20090116 4


Clathrin is a coat protein involved in vesicle budding from several membrane-bound compartments within the cell. Here we present an analysis of a temperature-sensitive (ts) mutant of clathrin heavy chain (CHC) in a multicellular animal. As expected Caenorhabditis elegans chc-1(b1025ts) mutant animals are defective in receptor-mediated endocytosis and arrest development soon after being shifted to the restrictive temperature. Steady-state clathrin levels in these mutants are reduced by more than  ...[more]

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