Dataset Information

JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

ABSTRACT: The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.

SUBMITTER: Zhang J

PROVIDER: S-EPMC2634810 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

Zhang Junjie J Nakamura Natsuko N Shimizu Yuko Y Liang Nathan N Liu Xiangan X Jakana Joanita J Marsh Michael P MP Booth Christopher R CR Shinkawa Takao T Nakata Munetaka M Chiu Wah W

Journal of structural biology 20080930 1

The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded parti ...[more]

PMID: 18926912

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Dataset Information

JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

Publications

JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

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