Unknown

Dataset Information

0

IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus.


ABSTRACT: BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator responsible for extreme radioresistance. METHODOLOGY/PRINCIPAL FINDINGS: Using plate assays, we showed that IrrE protected E. coli cells against salt shock and other abiotic stresses such as oxidative, osmotic and thermal shocks. Comparative proteomic analysis revealed that IrrE functions as a switch to regulate different sets of proteins such as stress responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins, and growth-related proteins in E. coli. We also used quantitative RT-PCR to investigate expression of nine selected stress-responsive genes in transgenic and wild-type Brassica napus plants. Transgenic B. napus plants expressing the IrrE protein can tolerate 350 mM NaCl, a concentration that inhibits the growth of almost all crop plants. CONCLUSIONS: Expression of IrrE, a global regulator for extreme radiation resistance in D. radiodurans, confers significantly enhanced salt tolerance in both E. coli and B. napus. We thus propose that the irrE gene might be used as a potentially promising transgene to improve abiotic stress tolerances in crop plants.

SUBMITTER: Pan J 

PROVIDER: S-EPMC2635966 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

altmetric image

Publications

IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus.

Pan Jie J   Wang Jin J   Zhou Zhengfu Z   Yan Yongliang Y   Zhang Wei W   Lu Wei W   Ping Shuzhen S   Dai Qilin Q   Yuan Menglong M   Feng Bin B   Hou Xiaoguang X   Zhang Ying Y   Ma Ruiqiang R   Liu Tingting T   Feng Lu L   Wang Lei L   Chen Ming M   Lin Min M  

PloS one 20090210 2


<h4>Background</h4>Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator re  ...[more]

Similar Datasets

| S-EPMC151961 | biostudies-literature
| S-EPMC6952347 | biostudies-literature
2022-08-29 | PXD027969 | Pride
| S-EPMC5552922 | biostudies-literature
| S-EPMC153069 | biostudies-literature
| S-EPMC3554781 | biostudies-literature
| S-EPMC4171365 | biostudies-literature
| S-EPMC5009958 | biostudies-literature
| S-EPMC2755865 | biostudies-literature
| S-EPMC9650054 | biostudies-literature