Ontology highlight
ABSTRACT:
SUBMITTER: Howarth M
PROVIDER: S-EPMC2637151 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Nature methods 20080420 5
We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monoval ...[more]