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Activity-dependent regulation of inhibitory synapse development by Npas4.

ABSTRACT: Neuronal activity regulates the development and maturation of excitatory and inhibitory synapses in the mammalian brain. Several recent studies have identified signalling networks within neurons that control excitatory synapse development. However, less is known about the molecular mechanisms that regulate the activity-dependent development of GABA (gamma-aminobutyric acid)-releasing inhibitory synapses. Here we report the identification of a transcription factor, Npas4, that plays a role in the development of inhibitory synapses by regulating the expression of activity-dependent genes, which in turn control the number of GABA-releasing synapses that form on excitatory neurons. These findings demonstrate that the activity-dependent gene program regulates inhibitory synapse development, and suggest a new role for this program in controlling the homeostatic balance between synaptic excitation and inhibition.

SUBMITTER: Lin Y

PROVIDER: S-EPMC2637532 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Activity-dependent regulation of inhibitory synapse development by Npas4.

Lin Yingxi Y Bloodgood Brenda L BL Hauser Jessica L JL Lapan Ariya D AD Koon Alex C AC Kim Tae-Kyung TK Hu Linda S LS Malik Athar N AN Greenberg Michael E ME

Nature 20080924 7217

Neuronal activity regulates the development and maturation of excitatory and inhibitory synapses in the mammalian brain. Several recent studies have identified signalling networks within neurons that control excitatory synapse development. However, less is known about the molecular mechanisms that regulate the activity-dependent development of GABA (gamma-aminobutyric acid)-releasing inhibitory synapses. Here we report the identification of a transcription factor, Npas4, that plays a role in the ...[more]

PMID: 18815592

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Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-05-24 | E-GEOD-55591 | biostudies-arrayexpress

Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons (array)

Project description:To identify the activity-induced gene expression programs in inhibitory and excitatory neurons, we analyzed RNA extracted from cultured E14 mouse MGE- and CTX-derived neurons (DIV 10) after these cultures were membrane-depolarized for 0, 1 and 6 hrs with 55mM extracellular KCl. To identify the gene programs regulated in these cells by the activity-induced early-response transcription factor Npas4, we repeated the same experiment in the MGE- and CTX-cultures lacking Npas4 (Npas4-KO). Littermate mouse E14 MGE- or CTX-derived neurons (WT or KO for Npas4) were cultured for 9 days, quieted overnight with TTX and AP-5 and then membrane-depolarized for 0, 1 or 6 hours by raising the extracellular KCl-concentration to 55mM. RNA was then extracted and analyzed using Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.

2015-06-04 | E-GEOD-55588 | biostudies-arrayexpress

Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-05-24 | GSE55591 | GEO

Novel mouse model reveals distinct activity-dependent and -independent contributions to synapse development.

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Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons (array)

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