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Identification of activity-induced Npas4-regulated genes in cortical inhibitory and excitatory neurons (array)

ABSTRACT: To identify the activity-induced gene expression programs in inhibitory and excitatory neurons, we analyzed RNA extracted from cultured E14 mouse MGE- and CTX-derived neurons (DIV 10) after these cultures were membrane-depolarized for 0, 1 and 6 hrs with 55mM extracellular KCl. To identify the gene programs regulated in these cells by the activity-induced early-response transcription factor Npas4, we repeated the same experiment in the MGE- and CTX-cultures lacking Npas4 (Npas4-KO). Littermate mouse E14 MGE- or CTX-derived neurons (WT or KO for Npas4) were cultured for 9 days, quieted overnight with TTX and AP-5 and then membrane-depolarized for 0, 1 or 6 hours by raising the extracellular KCl-concentration to 55mM. RNA was then extracted and analyzed using Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.

ORGANISM(S): Mus musculus

SUBMITTER: Ivo Spiegel 

PROVIDER: E-GEOD-55588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Npas4 regulates excitatory-inhibitory balance within neural circuits through cell-type-specific gene programs.

Spiegel Ivo I   Mardinly Alan R AR   Gabel Harrison W HW   Bazinet Jeremy E JE   Couch Cameron H CH   Tzeng Christopher P CP   Harmin David A DA   Greenberg Michael E ME  

Cell 20140501 5

The nervous system adapts to experience by inducing a transcriptional program that controls important aspects of synaptic plasticity. Although the molecular mechanisms of experience-dependent plasticity are well characterized in excitatory neurons, the mechanisms that regulate this process in inhibitory neurons are only poorly understood. Here, we describe a transcriptional program that is induced by neuronal activity in inhibitory neurons. We find that, while neuronal activity induces expressio  ...[more]

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