Ontology highlight
ABSTRACT:
SUBMITTER: Shaulsky G
PROVIDER: S-EPMC26391 | biostudies-literature | 1996 Dec
REPOSITORIES: biostudies-literature
Shaulsky G G Escalante R R Loomis W F WF
Proceedings of the National Academy of Sciences of the United States of America 19961201 26
We have found conditions for saturation mutagenesis by restriction enzyme mediated integration that result in plasmid tagging of disrupted genes. Using this method we selected for mutations in genes that act at checkpoints downstream of the intercellular signalling system that controls encapsulation in Dictyostelium discoideum. One of these genes, mkcA, is a member of the mitogen-activating protein kinase cascade family while the other, regA is a novel bipartite gene homologous to response regul ...[more]