Ontology highlight
ABSTRACT:
SUBMITTER: Maurel D
PROVIDER: S-EPMC2642604 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Maurel Damien D Comps-Agrar Laëtitia L Brock Carsten C Rives Marie-Laure ML Bourrier Emmanuel E Ayoub Mohammed Akli MA Bazin Hervé H Tinel Norbert N Durroux Thierry T Prézeau Laurent L Trinquet Eric E Pin Jean-Philippe JP
Nature methods 20080518 6
Cell-surface proteins are important in cell-cell communication. They assemble into heterocomplexes that include different receptors and effectors. Elucidation and manipulation of such protein complexes offers new therapeutic possibilities. We describe a methodology combining time-resolved fluorescence resonance energy transfer (FRET) with snap-tag technology to quantitatively analyze protein-protein interactions at the surface of living cells, in a high throughput-compatible format. Using this a ...[more]