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Identification and characterization of Class 1 integron resistance gene cassettes among Salmonella strains isolated from imported seafood.

ABSTRACT: A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridization of plasmids isolated from these strains, using a class 1 integron probe, showed that trimethoprim-sulfamethoxazole and streptomycin resistance genes were located on a megaplasmid in strain 629. Our study indicates that imported seafood could be a reservoir for Salmonella isolates resistant to multiple antibiotics.

SUBMITTER: Khan AA

PROVIDER: S-EPMC2643569 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Identification and characterization of Class 1 integron resistance gene cassettes among Salmonella strains isolated from imported seafood.

Khan Ashraf A AA Ponce Elizabeth E Nawaz M S MS Cheng Chorng-Ming CM Khan Junaid A JA West Christine S CS

Applied and environmental microbiology 20081212 4

A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridizatio ...[more]

PMID: 19074612

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