Ontology highlight
ABSTRACT:
SUBMITTER: Leyton JV
PROVIDER: S-EPMC2644405 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Leyton Jeffrey V JV Olafsen Tove T Sherman Mark A MA Bauer Karl B KB Aghajanian Patrick P Reiter Robert E RE Wu Anna M AM
Protein engineering, design & selection : PEDS 20081028 3
We have previously demonstrated preclinical in vivo targeting of prostate stem cell antigen (PSCA) using a humanized anti-PSCA 2B3 monoclonal antibody (mAb). However, humanization resulted in 5-fold loss of apparent affinity relative to the parental mAb (1 nM). In this study, diabodies (scFv dimers of 55 kDa) were generated from 2B3 including variants with different linker lengths as well as back-mutations to original murine residues to improve affinity. Parental 2B3 (p2B3) and back-mutated 2B3 ...[more]