Ontology highlight
ABSTRACT:
SUBMITTER: Zhao JH
PROVIDER: S-EPMC2644755 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Zhao Jie-hong JH Zhao De-gang DG
Journal of Zhejiang University. Science. B 20090201 2
We constructed an expression cassette of the organophosphorus pesticide degrading (opd) gene under the control of the E8 promoter. Then opd was transformed into tomato fruit using an agroinfiltration transient expression system. beta-Glucuronidase (GUS) staining, reverse transcription-polymerase chain reaction (RT-PCR), wavelength scanning, and fluorescent reaction were performed to examine the expression of the opd gene and the hydrolysis activity on coumaphos of organophosphorus hydrolase (OPH ...[more]