Ontology highlight
ABSTRACT:
SUBMITTER: Guo Q
PROVIDER: S-EPMC2647313 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Nucleic acids research 20090107 3
Here we have developed a sensitive DNA amplified detection method based on isothermal strand-displacement polymerization reaction. This method takes advantage of both the hybridization property of DNA and the strand-displacement property of polymerase. Importantly, we demonstrate that our method produces a circular polymerization reaction activated by the target, which essentially allows it to self-detect. Functionally, this DNA system consists of a hairpin fluorescence probe, a short primer and ...[more]