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Electron microscopy of whole cells in liquid with nanometer resolution.

ABSTRACT: Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.

SUBMITTER: de Jonge N

PROVIDER: S-EPMC2650183 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Electron microscopy of whole cells in liquid with nanometer resolution.

de Jonge N N Peckys D B DB Kremers G J GJ Piston D W DW

Proceedings of the National Academy of Sciences of the United States of America 20090121 7

Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness ...[more]

PMID: 19164524

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