Unknown

Dataset Information

0

Thirteen posttranslational modifications convert a 14-residue peptide into the antibiotic thiocillin.


ABSTRACT: The thiazolylpeptides are a family of >50 bactericidal antibiotics that block the initial steps of bacterial protein synthesis. Here, we report a biosynthetic gene cluster for thiocillin and establish that it, and by extension the whole class, is ribosomally synthesized. Remarkably, the C-terminal 14 residues of a 52-residue peptide precursor undergo 13 posttranslational modifications to give rise to thiocillin, making this antibiotic the most heavily posttranslationally-modified peptide known to date.

SUBMITTER: Wieland Brown LC 

PROVIDER: S-EPMC2650375 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Thirteen posttranslational modifications convert a 14-residue peptide into the antibiotic thiocillin.

Wieland Brown Laura C LC   Acker Michael G MG   Clardy Jon J   Walsh Christopher T CT   Fischbach Michael A MA  

Proceedings of the National Academy of Sciences of the United States of America 20090205 8


The thiazolylpeptides are a family of >50 bactericidal antibiotics that block the initial steps of bacterial protein synthesis. Here, we report a biosynthetic gene cluster for thiocillin and establish that it, and by extension the whole class, is ribosomally synthesized. Remarkably, the C-terminal 14 residues of a 52-residue peptide precursor undergo 13 posttranslational modifications to give rise to thiocillin, making this antibiotic the most heavily posttranslationally-modified peptide known t  ...[more]

Similar Datasets

| S-EPMC10524105 | biostudies-literature
| S-EPMC8689077 | biostudies-literature
| S-EPMC2860372 | biostudies-literature
| S-EPMC6035883 | biostudies-literature
| S-EPMC8348142 | biostudies-literature
| S-EPMC2397375 | biostudies-literature
| S-EPMC4713141 | biostudies-literature
| S-EPMC3981057 | biostudies-other
| S-EPMC4074372 | biostudies-literature
| S-EPMC3404735 | biostudies-literature