Ontology highlight
ABSTRACT:
SUBMITTER: Muto T
PROVIDER: S-EPMC2650456 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Muto Takanori T Tsuchiya Daisuke D Morikawa Kosuke K Jingami Hisato H
Acta crystallographica. Section F, Structural biology and crystallization communications 20090214 Pt 3
Metabotropic glutamate receptors (mGluRs) are involved in the regulation of many physiological and pathological processes in the central nervous system. The extracellular domain (ECD) of mGluR subtype 3 (mGluR3) was produced using the baculovirus expression system and purified from the culture medium. However, the recombinant protein showed heterogeneity in molecular weight on SDS-PAGE analysis. It was found that the unglycosylation of Asn414 significantly reduced the heterogeneity. Consequently ...[more]