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Crystallization and preliminary X-ray analysis of human liver alpha-enolase.

ABSTRACT: Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. alpha-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 72.85, b = 66.02, c = 79.43 A, beta = 94.54 degrees .

SUBMITTER: Wang J

PROVIDER: S-EPMC2650463 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray analysis of human liver alpha-enolase.

Wang Juan J Zhou Yan Feng YF Li Lan Fen LF Su Xiao Dong XD

Acta crystallographica. Section F, Structural biology and crystallization communications 20090226 Pt 3

Enolase is a multifunctional enzyme that plays important roles in many biological and disease processes. alpha-Enolase from human liver (hENO1) was expressed as a soluble protein and purified by affinity column chromatography and gel filtration. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 72.85, b = 66.02, c = 79.43 A, beta = 94.54 degrees . ...[more]

PMID: 19255486

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