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Cross-priming amplification for rapid detection of Mycobacterium tuberculosis in sputum specimens.


ABSTRACT: Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.

SUBMITTER: Fang R 

PROVIDER: S-EPMC2650920 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Cross-priming amplification for rapid detection of Mycobacterium tuberculosis in sputum specimens.

Fang Rendong R   Li Xia X   Hu Lin L   You Qimin Q   Li Jing J   Wu Jie J   Xu Peng P   Zhong Huayan H   Luo Ying Y   Mei Jian J   Gao Qian Q  

Journal of clinical microbiology 20081230 3


Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%. ...[more]

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