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Secondary replicative function of CD8+ T cells that had developed an effector phenotype.

ABSTRACT: Models of the differentiation of memory CD8+ T cells that replicate during secondary infections differ over whether such cells had acquired effector function during primary infections. We created a transgenic mouse line that permits mapping of the fate of granzyme B (gzmB)-expressing CD8+ T cells and their progeny by indelibly marking them with enhanced yellow fluorescent protein (EYFP). Virus-specific CD8+ T cells express gzmB within the first 2 days of a primary response to infection with influenza, without impairment of continued primary clonal expansion. On secondary infection, virus-specific CD8+ T cells that became EYFP+ during a primary infection clonally expand as well as all virus-specific CD8+ T cells. Thus, CD8+ T cells that have acquired an effector phenotype during primary infection may function as memory cells with replicative function.

SUBMITTER: Bannard O

PROVIDER: S-EPMC2653633 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Secondary replicative function of CD8+ T cells that had developed an effector phenotype.

Bannard Oliver O Kraman Matthew M Fearon Douglas T DT

Science (New York, N.Y.) 20090101 5913

Models of the differentiation of memory CD8+ T cells that replicate during secondary infections differ over whether such cells had acquired effector function during primary infections. We created a transgenic mouse line that permits mapping of the fate of granzyme B (gzmB)-expressing CD8+ T cells and their progeny by indelibly marking them with enhanced yellow fluorescent protein (EYFP). Virus-specific CD8+ T cells express gzmB within the first 2 days of a primary response to infection with infl ...[more]

PMID: 19164749

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