Ontology highlight
ABSTRACT:
SUBMITTER: Kulukian A
PROVIDER: S-EPMC2655205 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Kulukian Anita A Han Joo Seok JS Cleveland Don W DW
Developmental cell 20090101 1
Premature anaphase onset is prevented by the mitotic checkpoint through production of a "wait anaphase" inhibitor(s) that blocks recognition of cyclin B and securin by Cdc20-activated APC/C, an E3 ubiquitin ligase that targets them for destruction. Using physiologically relevant levels of Mad2, Bub3, BubR1, and Cdc20, we demonstrate that unattached kinetochores on purified chromosomes catalytically generate a diffusible Cdc20 inhibitor or inhibit Cdc20 already bound to APC/C. Furthermore, the ch ...[more]