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Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety.

ABSTRACT: The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.

SUBMITTER: Li M

PROVIDER: S-EPMC2655352 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety.

Li Minyong M Lin Na N Huang Zhen Z Du Lupei L Altier Craig C Fang Hao H Wang Binghe B

Journal of the American Chemical Society 20080903 38

The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides. ...[more]

PMID: 18763762

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