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Analysis of growth-phase regulated genes in Streptococcus agalactiae by global transcript profiling.

ABSTRACT: BACKGROUND: Bacteria employ multiple mechanisms to control gene expression and react to their constantly changing environment. Bacterial growth in rich laboratory medium is a dynamic process in which bacteria utilize nutrients from simple to complex and change physical properties of the medium, as pH, during the process. To determine which genes are differentially expressed throughout growth from mid log to stationary phase, we performed global transcript analysis. RESULTS: The S. agalactiae transcriptome is dynamic in response to growth conditions. Several genes and regulons involved in virulence factor production and utilization of alternate carbon sources were differentially expressed throughout growth. CONCLUSION: These data provide new information about the magnitude of plasticity of the S. agalactiae transcriptome and its adaptive response to changing environmental conditions. The resulting information will greatly assist investigators studying S. agalactiae physiology and pathogenesis.

SUBMITTER: Sitkiewicz I

PROVIDER: S-EPMC2660347 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Analysis of growth-phase regulated genes in Streptococcus agalactiae by global transcript profiling.

Sitkiewicz Izabela I Musser James M JM

BMC microbiology 20090210

<h4>Background</h4>Bacteria employ multiple mechanisms to control gene expression and react to their constantly changing environment. Bacterial growth in rich laboratory medium is a dynamic process in which bacteria utilize nutrients from simple to complex and change physical properties of the medium, as pH, during the process. To determine which genes are differentially expressed throughout growth from mid log to stationary phase, we performed global transcript analysis.<h4>Results</h4>The S. a ...[more]

PMID: 19208240

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Project description:Streptococcus agalactiae (group B Streptococcus; GBS) is a significant bacterial pathogen of neonates and an emerging pathogen of adults. Though transcriptional regulators are abundantly encoded on the GBS genome, their role in GBS pathogenesis is poorly understood. The mtaR gene encodes a putative LysR-type transcriptional regulator that is critical for the full virulence of GBS. Previous studies have shown that an mtaR- mutant transports methionine at reduced rates and grows poorly in normal human plasma not supplemented with methionine. The decreased virulence of the mtaR mutant was correlated with a methionine transport defect; however, no MtaR-regulated genes were identified.Microarray analysis of wild-type GBS and an mtaR mutant revealed differential expression of 12 genes, including 1 upregulated and 11 downregulated genes in the mtaR mutant. Among the downregulated genes, we identified a cluster of cotranscribed genes encoding a putative methionine transporter (metQ1NP) and peptidase (pdsM). The expression of four genes potentially involved in arginine transport (artPQ) and arginine biosynthesis (argGH) was downregulated and these genes localized to two transcriptional units. The virulence factor cspA, which encodes an extracellular protease, was downregulated. Additionally, the SAN_1255 locus, which putatively encodes a protein displaying similarity to plasminogen activators, was downregulated.To our knowledge, this is the first study to describe the global influence of MtaR on GBS gene expression. This study implicates the metQ1NP genes as encoding the MtaR-regulated methionine transporter, which may provide a mechanistic explanation for the methionine-dependent growth defect of the mtaR mutant. In addition to modulating the expression of genes involved in metabolism and amino acid transport, inactivation of mtaR affected the expression of other GBS genes implicated in pathogenesis. These findings suggest the possibility that MtaR may play a multifaceted role in GBS pathogenesis by regulating the expression of numerous genes.

Dataset Information

Analysis of growth-phase regulated genes in Streptococcus agalactiae by global transcript profiling.

Publications

Analysis of growth-phase regulated genes in Streptococcus agalactiae by global transcript profiling.

Similar Datasets

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