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ABSTRACT:
SUBMITTER: McLean KJ
PROVIDER: S-EPMC2662266 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
McLean Kirsty J KJ Carroll Paul P Lewis D Geraint DG Dunford Adrian J AJ Seward Harriet E HE Neeli Rajasekhar R Cheesman Myles R MR Marsollier Laurent L Douglas Philip P Smith W Ewen WE Rosenkrands Ida I Cole Stewart T ST Leys David D Parish Tanya T Munro Andrew W AW
The Journal of biological chemistry 20080924 48
Mycobacterium tuberculosis (Mtb) cytochrome P450 gene CYP121 is shown to be essential for viability of the bacterium in vitro by gene knock-out with complementation. Production of CYP121 protein in Mtb cells is demonstrated. Minimum inhibitory concentration values for azole drugs against Mtb H37Rv were determined, the rank order of which correlated well with Kd values for their binding to CYP121. Solution-state spectroscopic, kinetic, and thermodynamic studies and crystal structure determination ...[more]