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Daptomycin versus Friulimicin B: in-depth profiling of Bacillus subtilis cell envelope stress responses.

ABSTRACT: The related lipo(depsi)peptide antibiotics daptomycin and friulimicin B show great potential in the treatment of multiply resistant gram-positive pathogens. Applying genome-wide in-depth expression profiling, we compared the respective stress responses of Bacillus subtilis. Both antibiotics target envelope integrity, based on the strong induction of extracytoplasmic function sigma factor-dependent gene expression. The cell envelope stress-sensing two-component system LiaRS is exclusively and strongly induced by daptomycin, indicative of different mechanisms of action in the two compounds.

SUBMITTER: Wecke T

PROVIDER: S-EPMC2663105 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Daptomycin versus Friulimicin B: in-depth profiling of Bacillus subtilis cell envelope stress responses.

Wecke Tina T Zühlke Daniela D Mäder Ulrike U Jordan Sina S Voigt Birgit B Pelzer Stefan S Labischinski Harald H Homuth Georg G Hecker Michael M Mascher Thorsten T

Antimicrobial agents and chemotherapy 20090121 4

The related lipo(depsi)peptide antibiotics daptomycin and friulimicin B show great potential in the treatment of multiply resistant gram-positive pathogens. Applying genome-wide in-depth expression profiling, we compared the respective stress responses of Bacillus subtilis. Both antibiotics target envelope integrity, based on the strong induction of extracytoplasmic function sigma factor-dependent gene expression. The cell envelope stress-sensing two-component system LiaRS is exclusively and str ...[more]

PMID: 19164157

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Daptomycin stimulon in Bacillus subtilis W168

2008-12-10 | E-GEOD-13900 | biostudies-arrayexpress

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Project description:Investigation of the whole genome expression level changes in phosphate limited Bacillus subtilis wild-type and delta-phoPR cells Investigation of the whole genome expression level changes of wild-type and delta-phoPR Bacills subtilis cells comparing high and low phosphate medium For each sample analyzed in this study 3 technical replicates were performed. 3 different samples were taken for wild-type cell and delta-phoPR cell, respectively. Samples were taken from exponentially growing cells in high and low phosphate medium as well as from phosphate-limited cells.

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