Ontology highlight
ABSTRACT:
SUBMITTER: Pei XY
PROVIDER: S-EPMC2663715 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Pei Xue Yuan XY Titman Christopher M CM Frank René A W RA Leeper Finian J FJ Luisi Ben F BF
Structure (London, England : 1993) 20081201 12
The pyruvate dehydrogenase multienzyme assembly (PDH) generates acetyl coenzyme A and reducing equivalents from pyruvate in a multiple-step process that is a nexus of central metabolism. We report crystal structures of the Geobacillus stearothermophilus PDH E1p subunit with ligands that mimic the prereaction complex and the postdecarboxylation product. The structures implicate residues that help to orient substrates, nurture intermediates, and organize surface loops so that they can engage a mob ...[more]