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Structure of Debaryomyces castellii CBS 2923 phytase.

ABSTRACT: Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.

SUBMITTER: Ragon M

PROVIDER: S-EPMC2664750 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Structure of Debaryomyces castellii CBS 2923 phytase.

Ragon M M Hoh F F Aumelas A A Chiche L L Moulin G G Boze H H

Acta crystallographica. Section F, Structural biology and crystallization communications 20090325 Pt 4

Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and ...[more]

PMID: 19342770

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