Phytase
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB12987 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1306540_1.fastq.gz | Fastqsanger.gz | |||
| ERR1306540_2.fastq.gz | Fastqsanger.gz | |||
| ERR1307060_1.fastq.gz | Fastqsanger.gz | |||
| ERR1307060_2.fastq.gz | Fastqsanger.gz | |||
| ERR1307070_1.fastq.gz | Fastqsanger.gz |
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Project description:Development of Pectoralis major has been investigated through gene expression analysis in comparing animals receiving a restricted diet in P and Ca (NC), a normal diet with sufficient level of P and Ca (PC), and a restricted diet supplemented with phytase (Phy1000). We used microarrays (ChiGene-1_0-st) to evaluate gene expression underlying pathways affectd by the diet and/or the phytase supplementation and to identify classes of differentially expressed genes.
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Project description:Five experimental diets were prepared with equal levels of protein (48% crude protein) and lipid (12% crude lipid): a diet based on fishmeal (referred to as Group FM), a diet containing 7% β-conglycinin (referred to as Group 7S), a diet containing 10% glycinin (referred to as Group 11S), a β-conglycinin diet supplemented with 2% alanine-glutamine (referred to as Group CCgGln), and glycinin diet supplemented with 2% alanine-glutamine (referred to as Group GcGln). Healthy hybrid groupers (8.50 ± 0.01 g) were fed each diet for 8 weeks. After the feeding trial finished, distal intestine (DI) samples from the three groups were obtained to determine proteome.
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Project description:Intense selective breeding of broiler breeds of chickens has resulted in suboptimal egg production in broiler breeder hens. Ad libitum feeding which leads to excessive and disorganized follicular growth exacerbates this reproductive phenotype. One strategy used to improve broiler breeder hen reproductive efficiency is restricted feeding. In this study, we sought to identify transcriptional changes which translate level of dietary intake to increased follicle selection. Broiler breeder hens were raised according to commercial guidelines until 28 weeks of age and then randomly assigned to an ad libitum diet (FF) or continued on a restricted diet (RF) for 6 weeks. Following dietary treatment, granulosa cells from growing 6-8 mm follicles from FF hens (n=3) and RF hens (n=3) were collected, RNA was extracted, and samples were processed for RNA-sequencing on Illumina NextSeq 500. Transcriptomes of granulosa cells from 6-8 mm follicles were sequenced to identify transcriptional differences in the population from which follicles are selected into the preovulatory stage. FastQ files were first processed through trim-galore and reads were aligned to the Galgal6 genome using the RNA-seq aligner, STAR. A cluster analysis using hclust in R identified a FF sample as an outlier and this sample was removed from the analysis. Differential expression analysis was conducted using DeSEQ2 and resulted in 350 differentially expressed genes. Several genes involved in follicle selection were upregulated in prehierarchal follicles of FF hens, suggesting an effect of dietary treatment at early stages in follicle development.
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Project description:Illumina sequencing of chicken microbiome fed a phytase diet
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Project description:The family concerns 44 offspring of the sire BC1 n°1998 (produced from a F1 male obtained by-intercrossing two fat (FL) and lean (LL) meat-type chicken lines that divergently selected on fatness [Leclercq et al 1980] mated with 8 LL females. Hepatic transcriptomic profiles were obtained using a chicken 20K oligochips (ARK-genomics). The 44 chickens were fed ad libitum using a conventional starter diet (0-3 weeks: 12.8 MJ of metabolizable energy) and then a growing broiler diet (4-9 weeks: 13.0 Mj of metabolizable energy). Light/dark periods were 24h light for the first 2 days, then 14h light/10h night up to slaughtering. At 4 weeks of age, blood samples were collected for DNA extraction and genotyping. At 9 weeks of age, the birds were fed ad libitum for 4 hours minimum after overnight fasting and then weighed and sacrificed by electrical stunning in the experimental processing plant (Station de Recherches Avicoles, Inra, Nouzilly, France). Following sacrifice, livers were collected, quickly frozen in liquid nitrogen and stored at −80°C until RNA extraction for transcriptome analyses
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