Ontology highlight
ABSTRACT:
SUBMITTER: Bezsudnova EY
PROVIDER: S-EPMC2664762 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Bezsudnova E Y EY Kovalchuk M V MV Mardanov A V AV Poliakov K M KM Popov V O VO Ravin N V NV Skryabin K G KG Smagin V A VA Stekhanova T N TN Tikhonova T V TV
Acta crystallographica. Section F, Structural biology and crystallization communications 20090325 Pt 4
DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging-drop vapour-diffusion method employing 35%(v/v) Tacsimate pH 7.0 as a precipitant and diffracted X-rays to 3.09 A resolution. They bel ...[more]