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Borrelia burgdorferi EbfC defines a newly-identified, widespread family of bacterial DNA-binding proteins.


ABSTRACT: The Lyme disease spirochete, Borrelia burgdorferi, encodes a novel type of DNA-binding protein named EbfC. Orthologs of EbfC are encoded by a wide range of bacterial species, so characterization of the borrelial protein has implications that span the eubacterial kingdom. The present work defines the DNA sequence required for high-affinity binding by EbfC to be the 4 bp broken palindrome GTnAC, where 'n' can be any nucleotide. Two high-affinity EbfC-binding sites are located immediately 5' of B. burgdorferi erp transcriptional promoters, and binding of EbfC was found to alter the conformation of erp promoter DNA. Consensus EbfC-binding sites are abundantly distributed throughout the B. burgdorferi genome, occurring approximately once every 1 kb. These and other features of EbfC suggest that this small protein and its orthologs may represent a distinctive type of bacterial nucleoid-associated protein. EbfC was shown to bind DNA as a homodimer, and site-directed mutagenesis studies indicated that EbfC and its orthologs appear to bind DNA via a novel alpha-helical 'tweezer'-like structure.

SUBMITTER: Riley SP 

PROVIDER: S-EPMC2665219 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Borrelia burgdorferi EbfC defines a newly-identified, widespread family of bacterial DNA-binding proteins.

Riley Sean P SP   Bykowski Tomasz T   Cooley Anne E AE   Burns Logan H LH   Babb Kelly K   Brissette Catherine A CA   Bowman Amy A   Rotondi Matthew M   Miller M Clarke MC   DeMoll Edward E   Lim Kap K   Fried Michael G MG   Stevenson Brian B  

Nucleic acids research 20090210 6


The Lyme disease spirochete, Borrelia burgdorferi, encodes a novel type of DNA-binding protein named EbfC. Orthologs of EbfC are encoded by a wide range of bacterial species, so characterization of the borrelial protein has implications that span the eubacterial kingdom. The present work defines the DNA sequence required for high-affinity binding by EbfC to be the 4 bp broken palindrome GTnAC, where 'n' can be any nucleotide. Two high-affinity EbfC-binding sites are located immediately 5' of B.  ...[more]

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