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Proteomic and genomic analysis of PITX2 interacting and regulating networks.

ABSTRACT: Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in mass spectrometry analysis. We also found that overexpression of PITX2 upregulated 868 genes (2-25-fold) and downregulated 191 genes (2-15-fold) in DNA microarray analysis. These data provide an insightful perspective for further studying PITX2 function and mechanism of action.

SUBMITTER: Huang Y

PROVIDER: S-EPMC2667122 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Proteomic and genomic analysis of PITX2 interacting and regulating networks.

Huang Yue Y Huang Kan K Boskovic Goran G Dementieva Yulia Y Denvir James J Primerano Donald A DA Zhu Guo-Zhang GZ

FEBS letters 20090125 4

Pituitary homeobox 2 (PITX2) is a homeodomain transcription factor that has a substantial role in cell proliferation and differentiation in various tissues. In this report, we have conducted a systematic study, using proteomic and genomic approaches, to characterize PITX2-interacting proteins and PITX2-regulating genes. We identified four novel PITX2-associated protein partners Y box binding factor-1, heterogeneous ribonucleoprotein K, nucleolin and heterogeneous nuclear ribonucleoprotein U in m ...[more]

PMID: 19174163

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