Auto-hydroxylation of FIH-1: an Fe(ii), alpha-ketoglutarate-dependent human hypoxia sensor.
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ABSTRACT:
SUBMITTER: Chen YH
PROVIDER: S-EPMC2668816 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Publications
Auto-hydroxylation of FIH-1: an Fe(ii), alpha-ketoglutarate-dependent human hypoxia sensor.
Chen Yuan-Han YH Comeaux Lindsay M LM Eyles Stephen J SJ Knapp Michael J MJ
Chemical communications (Cambridge, England) 20080811 39
HIF-asparaginyl hydroxylase (FIH-1) normally couples O(2)-activation to hydroxylation of Asn(803) on the alpha-subunit of the hypoxia-inducible factor (HIFalpha), a key step in pO(2) sensing; in the absence of HIFalpha, O(2)-activation becomes uncoupled, leading to self-hydroxylation at Trp(296) and a purple Fe(iii)-O-Trp chromophore-this alternative reactivity may affect human hypoxia sensing. ...[more]
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