Ultra low-dose radiation: stress responses and impacts using rice as a grass model.

ABSTRACT: We report molecular changes in leaves of rice plants (Oryza sativa L. - reference crop plant and grass model) exposed to ultra low-dose ionizing radiation, first using contaminated soil from the exclusion zone around Chernobyl reactor site. Results revealed induction of stress-related marker genes (Northern blot) and secondary metabolites (LC-MS/MS) in irradiated leaf segments over appropriate control. Second, employing the same in vitro model system, we replicated results of the first experiment using in-house fabricated sources of ultra low-dose gamma (gamma) rays and selected marker genes by RT-PCR. Results suggest the usefulness of the rice model in studying ultra low-dose radiation response/s.

SUBMITTER: Rakwal R 

PROVIDER: S-EPMC2672026 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature