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Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.


ABSTRACT: The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.

SUBMITTER: Korkhin Y 

PROVIDER: S-EPMC2675907 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

Korkhin Yakov Y   Unligil Ulug M UM   Littlefield Otis O   Nelson Pamlea J PJ   Stuart David I DI   Sigler Paul B PB   Bell Stephen D SD   Abrescia Nicola G A NG  

PLoS biology 20090505 5


The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryoti  ...[more]

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