Unknown

Dataset Information

0

The structural role of the zinc ion can be dispensable in prokaryotic zinc-finger domains.


ABSTRACT: The recent characterization of the prokaryotic Cys(2)His(2) zinc-finger domain, identified in Ros protein from Agrobacterium tumefaciens, has demonstrated that, although possessing a similar zinc coordination sphere, this domain is structurally very different from its eukaryotic counterpart. A search in the databases has identified approximately 300 homologues with a high sequence identity to the Ros protein, including the amino acids that form the extensive hydrophobic core in Ros. Surprisingly, the Cys(2)His(2) zinc coordination sphere is generally poorly conserved in the Ros homologues, raising the question of whether the zinc ion is always preserved in these proteins. Here, we present a functional and structural study of a point mutant of Ros protein, Ros(56-142)C82D, in which the second coordinating cysteine is replaced by an aspartate, 5 previously-uncharacterized representative Ros homologues from Mesorhizobium loti, and 2 mutants of the homologues. Our results indicate that the prokaryotic zinc-finger domain, which in Ros protein tetrahedrally coordinates Zn(II) through the typical Cys(2)His(2) coordination, in Ros homologues can either exploit a CysAspHis(2) coordination sphere, previously never described in DNA binding zinc finger domains to our knowledge, or lose the metal, while still preserving the DNA-binding activity. We demonstrate that this class of prokaryotic zinc-finger domains is structurally very adaptable, and surprisingly single mutations can transform a zinc-binding domain into a nonzinc-binding domain and vice versa, without affecting the DNA-binding ability. In light of our findings an evolutionary link between the prokaryotic and eukaryotic zinc-finger domains, based on bacteria-to-eukaryota horizontal gene transfer, is discussed.

SUBMITTER: Baglivo I 

PROVIDER: S-EPMC2678482 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

The structural role of the zinc ion can be dispensable in prokaryotic zinc-finger domains.

Baglivo Ilaria I   Russo Luigi L   Esposito Sabrina S   Malgieri Gaetano G   Renda Mario M   Salluzzo Antonio A   Di Blasio Benedetto B   Isernia Carla C   Fattorusso Roberto R   Pedone Paolo V PV  

Proceedings of the National Academy of Sciences of the United States of America 20090415 17


The recent characterization of the prokaryotic Cys(2)His(2) zinc-finger domain, identified in Ros protein from Agrobacterium tumefaciens, has demonstrated that, although possessing a similar zinc coordination sphere, this domain is structurally very different from its eukaryotic counterpart. A search in the databases has identified approximately 300 homologues with a high sequence identity to the Ros protein, including the amino acids that form the extensive hydrophobic core in Ros. Surprisingly  ...[more]

Similar Datasets

| S-EPMC9569694 | biostudies-literature
| S-EPMC7283297 | biostudies-literature
| S-EPMC7913770 | biostudies-literature
| S-EPMC4519095 | biostudies-literature
| S-EPMC6875160 | biostudies-literature
| S-EPMC3729454 | biostudies-literature
| S-EPMC7958900 | biostudies-literature
2022-03-01 | E-MTAB-11484 | biostudies-arrayexpress
2021-01-05 | E-MTAB-9236 | biostudies-arrayexpress
| S-EPMC7549681 | biostudies-literature