Ontology highlight
ABSTRACT:
SUBMITTER: Boeneman K
PROVIDER: S-EPMC2679970 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20090301 11
We demonstrate the use of a hybrid fluorescent protein semiconductor quantum dot (QD) sensor capable of specifically monitoring caspase 3 proteolytic activity. mCherry monomeric red fluorescent protein engineered to express an N-terminal caspase 3 cleavage site was ratiometrically self-assembled to the surface of QDs using metal-affinity coordination. The proximity of the fluorescent protein to the QD allows it to function as an efficient fluorescence resonance energy transfer acceptor. Addition ...[more]