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Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.

ABSTRACT: Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.

SUBMITTER: Andrasfalvy BK

PROVIDER: S-EPMC4245189 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology.

Andrásfalvy Bertalan K BK Galiñanes Gregorio L GL Huber Daniel D Barbic Mladen M Macklin John J JJ Susumu Kimihiro K Delehanty James B JB Huston Alan L AL Makara Judit K JK Medintz Igor L IL

Nature methods 20141019 12

Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified ra ...[more]

PMID: 25326662

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