A model for the regulatory network controlling the dynamics of kinetochore microtubule plus-ends and poleward flux in metaphase.

ABSTRACT: Tight regulation of kinetochore microtubule dynamics is required to generate the appropriate position and movement of chromosomes on the mitotic spindle. A widely studied but mysterious aspect of this regulation occurs during metaphase when polymerization of kinetochore microtubule plus-ends is balanced by depolymerization at their minus-ends. Thus, kinetochore microtubules maintain a constant net length, allowing chromosomes to persist at the spindle equator, but consist of tubulin subunits that continually flux toward spindle poles. Here, we construct a feasible network of regulatory proteins for controlling kinetochore microtubule plus-end dynamics, which was combined with a Monte Carlo algorithm to simulate metaphase tubulin flux. We also test the network model by combining it with a f

SUBMITTER: Fernandez N 

PROVIDER: S-EPMC2683096 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature