Ontology highlight
ABSTRACT:
SUBMITTER: Lippow SM
PROVIDER: S-EPMC2685105 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Lippow Shaun M SM Aha Patti M PM Parker Matthew H MH Blake William J WJ Baynes Brian M BM Lipovsek Dasa D
Nucleic acids research 20090320 9
Type IIS restriction endonucleases cleave DNA outside their recognition sequences, and are therefore particularly useful in the assembly of DNA from smaller fragments. A limitation of type IIS restriction endonucleases in assembly of long DNA sequences is the relative abundance of their target sites. To facilitate ligation-based assembly of extremely long pieces of DNA, we have engineered a new type IIS restriction endonuclease that combines the specificity of the homing endonuclease I-SceI with ...[more]