Ontology highlight
ABSTRACT:
SUBMITTER: Hondorp ER
PROVIDER: S-EPMC2687158 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Hondorp Elise R ER Matthews Rowena G RG
Journal of bacteriology 20090313 10
Cobalamin-independent methionine synthase (MetE) catalyzes the final step in Escherichia coli methionine biosynthesis but is inactivated under oxidative conditions, triggering a methionine deficiency. This study demonstrates that the mutation of MetE cysteine 645 to alanine completely eliminates the methionine auxotrophy imposed by diamide treatment, suggesting that modulation of MetE activity via cysteine 645 oxidation has significant physiological consequences for oxidatively stressed cells. ...[more]