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Role of GerKB in germination and outgrowth of Clostridium perfringens spores.

ABSTRACT: Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.

SUBMITTER: Paredes-Sabja D

PROVIDER: S-EPMC2687284 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Role of GerKB in germination and outgrowth of Clostridium perfringens spores.

Paredes-Sabja Daniel D Setlow Peter P Sarker Mahfuzur R MR

Applied and environmental microbiology 20090410 11

Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slo ...[more]

PMID: 19363077

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