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Solution-phase synthesis of pyrrole-imidazole polyamides.

ABSTRACT: Pyrrole-imidazole polyamides are DNA-binding molecules that are programmable for a large repertoire of DNA sequences. Typical syntheses of this class of heterocyclic oligomers rely on solid-phase methods. Solid-phase methodologies offer rapid assembly on a micromole scale sufficient for biophysical characterizations and cell culture studies. In order to produce gram-scale quantities necessary for efficacy studies in animals, polyamides must be readily synthesized in solution. An 8-ring hairpin polyamide 1, which targets the DNA sequence 5'-WGWWCW-3', was chosen for our synthesis studies as this oligomer exhibits androgen receptor antagonism in cell culture models of prostate cancer. A convergent solution-phase synthesis of 1 from a small set of commercially available building blocks is presented which highlights principles for preparing gram quantities of pyrrole-imidazole oligomers with minimal chromatography.

SUBMITTER: Chenoweth DM

PROVIDER: S-EPMC2689157 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Solution-phase synthesis of pyrrole-imidazole polyamides.

Chenoweth David M DM Harki Daniel A DA Dervan Peter B PB

Journal of the American Chemical Society 20090501 20

Pyrrole-imidazole polyamides are DNA-binding molecules that are programmable for a large repertoire of DNA sequences. Typical syntheses of this class of heterocyclic oligomers rely on solid-phase methods. Solid-phase methodologies offer rapid assembly on a micromole scale sufficient for biophysical characterizations and cell culture studies. In order to produce gram-scale quantities necessary for efficacy studies in animals, polyamides must be readily synthesized in solution. An 8-ring hairpin p ...[more]

PMID: 19413320

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| S-EPMC8483358 | biostudies-literature

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